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![Determining Kd With an Alpha Assay | Application Support Knowledgebase | Lab Products & Services | PerkinElmer Determining Kd With an Alpha Assay | Application Support Knowledgebase | Lab Products & Services | PerkinElmer](https://resources.perkinelmer.com/corporate/images/cheng_prusoff_ask_tcm137-191721.jpg)
Determining Kd With an Alpha Assay | Application Support Knowledgebase | Lab Products & Services | PerkinElmer
![SOLVED: Use the graph to estimate the dissociation constant, Kd, and calculate the association constant, Ka, of the ligand. 0.75 0.25 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 [L] (M) Kd = [M] Ka = 10 M-1 SOLVED: Use the graph to estimate the dissociation constant, Kd, and calculate the association constant, Ka, of the ligand. 0.75 0.25 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 [L] (M) Kd = [M] Ka = 10 M-1](https://cdn.numerade.com/ask_images/46fc654ccce642daa883f2786866e8fe.jpg)