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Why use M13F tailed primers for PCR and sequencing? What causes the real benefit in contrast to the usual primer? | ResearchGate
![SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure](https://cdn.numerade.com/ask_images/ac81f6690f8a4daaa48795d147bc881b.jpg)
SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure
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An alternative genotyping method using dye-labeled universal primer to reduce unspecific amplifications | Molecular Biology Reports
102-395-900 Technical overview: Multiplexed amplicon library preparation using SMRTbell Prep kit 3.0
![PCR primer cocktail components and corresponding sequences. M13 tails... | Download Scientific Diagram PCR primer cocktail components and corresponding sequences. M13 tails... | Download Scientific Diagram](https://www.researchgate.net/publication/51119782/figure/tbl2/AS:842507667636226@1577880889952/PCR-primer-cocktail-components-and-corresponding-sequences-M13-tails-are-also-listed.png)
PCR primer cocktail components and corresponding sequences. M13 tails... | Download Scientific Diagram
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